What is the toxicity of the fungicide, and what are the detection methods? China's pesticide network Xiaobian has compiled three major toxicity detection methods for fungicides from the network:
1) Spore germination method: Spray different liquid medicine on the surface of the slide or the plate, quantitatively add the spore suspension, and after the contact of the liquid, the percentage of spore germination is examined by a certain culture time.
2) Inhibition zone method: Mix the suspension of pathogen spores or hyphae with the agar medium, and after condensing, place a circular filter paper (diameter 6 mm) that is sterilized and has different concentrations of liquid medicine on the plane of the medium. Left and right), after a certain period of incubation at a fixed temperature, due to the diffusion of the drug solution, the growth of the pathogen is inhibited, that is, a "suppression circle" is formed. The size of the suppression ring was measured to compare the virulence of the biocide .
3) Growth rate measurement method: adding the chemical solution to the agar medium, condensing and collecting the bacteria, after 24-48 hours, observing the growth of the colony, calculating the growth rate, and the growth rate of the control group containing no drug Comparison.
The virulence of bactericides: the insecticidal or bactericidal virulence of the agent, commonly referred to as “lethal to mediumâ€, which means that the dose required to kill half (50%) of the biological population (median lethal dose, mg/kg) Abbreviated as LD50. If the concentration represents a dose, it is "lethal concentration", abbreviated as LC50. The bactericide is expressed as ED50 or EC50, ie the dose or concentration required to inhibit 50% spore germination.
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